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Related Experiment Videos

General RNA binding proteins render translation cap dependent

Y V Svitkin1, L P Ovchinnikov, G Dreyfuss

  • 1Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada.

The EMBO Journal
|December 16, 1996
PubMed
Summary
This summary is machine-generated.

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General RNA binding proteins are crucial for cap-dependent translation initiation. Adding these proteins to rabbit reticulocyte lysate makes translation reliant on the mRNA cap structure, preventing internal initiation.

Area of Science:

  • Molecular Biology
  • Gene Expression Regulation

Background:

  • Translation initiation in rabbit reticulocyte lysate is often independent of the mRNA m7G cap structure and cap-binding protein eIF-4E.
  • Spurious internal initiation sites are frequently utilized in this system.

Purpose of the Study:

  • To investigate the role of general RNA binding proteins in cap-dependent translation.
  • To determine if these proteins can restore cap dependency to translation extracts.

Main Methods:

  • Addition of purified general RNA binding proteins (hnRNP A1, La, PTB, p50) to rabbit reticulocyte lysate.
  • Comparison of translation efficiency for capped versus uncapped mRNAs in the presence and absence of these proteins.

Main Results:

  • General RNA binding proteins rendered translation cap-dependent in the lysate.

Related Experiment Videos

  • These proteins strongly inhibited uncapped mRNA translation while leaving capped mRNA translation unaffected.

    • Conclusions:

    • General mRNA binding proteins promote cap-mediated ribosome binding at the 5' end.
    • These proteins play a role in preventing aberrant translation initiation at internal sites.