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YB-3 substitutes YB-1 in global mRNA binding.

D N Lyabin1, I A Eliseeva1, E A Smolin1

  • 1Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia.

RNA Biology
|January 17, 2020
PubMed
Summary
This summary is machine-generated.

Y-box binding protein 1 (YB-1) inhibits translation of many mRNAs. In YB-1-null cells, YB-3 compensates by binding mRNAs and inhibiting their translation, suggesting YB-3 acts as a YB-1 substitute.

Keywords:
RIP-SeqRibo-SeqYB-1YB-3YBX1YBX3translational control

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Y-box binding proteins (YBPs) are ancient DNA/RNA-binding proteins.
  • Y-box binding protein 1 (YB-1) is abundant in somatic cells and regulates proliferation, differentiation, and stress response.
  • YB-1 is known to bind mRNAs and influence translation.

Purpose of the Study:

  • To investigate the global impact of YB-1 on mRNA binding and translation.
  • To elucidate the role of YB-3 in the absence of YB-1.
  • To understand the functional relationship between YB-1 and YB-3 in translational control.

Main Methods:

  • Ribosome profiling (Ribo-Seq) to analyze translation.
  • RNA immunoprecipitation sequencing (RIP-Seq) to identify bound mRNAs.
  • Gene knockout studies (YBX1 knockout) to assess YB-1's function.

Main Results:

  • YB-1 binds numerous mRNAs and globally inhibits their translation.
  • YBX1 knockout causes minor gene expression changes, primarily due to altered RNA abundance.
  • YB-3 mRNA translation is enhanced in YB-1-null cells, indicating negative regulation by YB-1.
  • YB-3 binds a similar set of mRNAs as YB-1, and its binding is enhanced in YB-1-null cells.
  • YB-3 binding leads to global translation reduction of bound mRNAs in YB-1-null cells.

Conclusions:

  • YB-1 globally inhibits translation of a wide range of mRNAs.
  • YB-3 can substitute for YB-1 in mRNA binding and global translational control.
  • YB-3 synthesis is under negative control by YB-1.