Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cations in human rhinoviruses

R Zhao1, A T Hadfield, M J Kremer

  • 1Department of Biological Sciences, Purdue University, West Lafayette, Indiana, 47907-1392, USA.

Virology
|January 6, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optically controllable THz chiral metamaterials.

Optics express·2014
Same author

Altered structural covariance of the striatum in functional dyspepsia patients.

Neurogastroenterology and motility·2014
Same author

Group housing during gestation affects the behaviour of sows and the physiological indices of offspring at weaning.

Animal : an international journal of animal bioscience·2014
Same author

A novel flow-diverting device (Tubridge) for the treatment of 28 large or giant intracranial aneurysms: a single-center experience.

AJNR. American journal of neuroradiology·2014
Same author

Characterization and full genome sequence analysis of a Chinese isolate of tomato chlorosis virus.

Acta virologica·2014
Same author

Large magnetoelectric coupling in Co4Nb2O9.

Scientific reports·2014
Same journal

Unveiling the hidden virome of Sclerotinia sclerotiorum: New tools and discoveries in mycovirus detection.

Virology·2026
Same journal

ASFV pDP238L negatively regulates type I interferon production via inhibiting the methylation of TBK1.

Virology·2026
Same journal

Divergent poxvirus identified in a non-native black rat from Madagascar.

Virology·2026
Same journal

Genomic and functional characterization of Bacillus phage BCE1 targeting a key gut bacterium in Aedes albopictus larvae.

Virology·2026
Same journal

Viral etiology of orogenital papillomatosis and squamous cell carcinoma in bottlenose dolphins in the southeastern United States.

Virology·2026
Same journal

Orthoflaviviruses in the modern era: Challenges and breakthroughs.

Virology·2026
See all related articles

Rhinoviruses contain an EGTA-chelatable ion, possibly calcium or zinc, near their fivefold axes. This cation may influence viral stability but its removal alone does not cause disassembly.

Area of Science:

  • Structural Biology
  • Virology
  • Biochemistry

Background:

  • Crystal structures of rhinoviruses consistently show uninterpreted electron density near the fivefold axis.
  • This density was previously assumed to be a calcium ion (Ca2+) due to its location and presence in crystallization solutions.

Purpose of the Study:

  • To identify the nature of the uninterpreted electron density in rhinovirus crystal structures.
  • To investigate the role of this ion in rhinovirus stability and disassembly.

Main Methods:

  • Difference electron density mapping was performed on EGTA-treated crystals of human rhinovirus 14 (HRV14) and HRV16.
  • Coordination geometry of the ion was analyzed in HRV3, HRV14, HRV1A, and HRV16.

Main Results:

Related Experiment Videos

  • The uninterpreted electron density represents an EGTA-chelatable ion.
  • Analysis suggests the ion is Ca2+ in HRV3, HRV14, and HRV1A, but potentially zinc (Zn2+) in HRV16.
  • The presence of this cation may be involved in regulating rhinovirus stability.

Conclusions:

  • The identified ion plays a role in rhinovirus stability.
  • However, the mere absence of this cation is insufficient to trigger viral disassembly.