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Strongly reduced phage Qbeta replication, but normal phage MS2 replication in an Escherichia coli K12 mutant with
1Institut für Molekularbiologie Abt. I, Universität Zürich, Zürich, 8093, Switzerland.
Virology
|January 6, 1997
Abstract:
RNA phage production was observed in Escherichia coli hfq mutant strains transformed with plasmids containing genomic cDNA of phages Qbeta or MS2. Qbeta production was reduced 5000-fold in an insertional mutant containing an omega cassette in the middle of hfq, but was unaffected in a mutant with the cassette near the end of hfq. The insertions had no effect on MS2 replication.