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Related Experiment Videos

Regulatory volume decrease and associated osmolyte fluxes in cerebellar granule neurons are calcium independent

J Morán1, S Morales-Mulia, A Hernández-Cruz

  • 1Department of Neuroscience, Institute of Cell Physiology, National University of Mexico, Mexico City.

Journal of Neuroscience Research
|January 15, 1997
PubMed
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Calcium (Ca) is not a signal for regulatory volume decrease (RVD) in neurons. Studies show that blocking Ca channels or release does not inhibit RVD or osmolyte release, indicating Ca

Area of Science:

  • Cellular Physiology
  • Neuroscience
  • Ion Transport

Background:

  • Regulatory volume decrease (RVD) is a critical cellular process for maintaining cell volume homeostasis.
  • Calcium ions (Ca) are often implicated as intracellular signaling molecules in various cellular responses.
  • The specific role of Ca in mediating RVD in neurons remains incompletely understood.

Purpose of the Study:

  • To investigate the potential role of extracellular and intracellular Ca as a transduction signal for RVD in neurons.
  • To examine the effects of Ca removal, channel blockers, and release inhibitors on RVD and osmolyte efflux during cell swelling.

Main Methods:

  • Assessed RVD and the efflux of chloride (Cl) and taurine using radioisotopes (125I and [3H]taurine) in response to osmotic swelling.

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  • Utilized various Ca chelators (EGTA, BAPTA), Ca channel blockers (Cd, Co, La, Gd, verapamil, diltiazem, dihydropyridines), and endoplasmic reticulum Ca release inhibitors (dantrolene, ryanodine, TMB-8).
  • Measured intracellular Ca ([Ca]i) changes using fura-2 during cell swelling.

    • Main Results:

    • External Ca removal and most Ca channel blockers did not affect RVD or osmolyte fluxes.
    • Specific dihydropyridines (nimodipine, nitrendipine) inhibited RVD and osmolyte fluxes independently of extracellular Ca.
    • Inhibitors of intracellular Ca release and permeable Ca chelators did not affect RVD or osmolyte fluxes, even when swelling-induced [Ca]i increases were blocked.

    Conclusions:

    • Extracellular Ca is not required for RVD or osmolyte release in neurons.
    • The observed increase in cytosolic Ca during swelling is likely a coincidental event and not a direct signal for RVD.
    • Calcium ions are unlikely to function as a primary transduction signal for regulatory volume decrease in neuronal cells.