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Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX
1Department of Immunology, National Institute of Health, Shinjuku-ku, Tokyo, Japan.
Abstract:
Translin is a DNA binding protein which specifically binds to consensus sequences at breakpoint junctions of chromosomal translocations in many cases of lymphoid malignancies. To investigate its functional significance at such recombination hotspots, we examined whether Translin interacts with other proteins using a yeast two-hybrid system and identified an associated 33 kd protein partner, TRAX, with extensive amino acid homology. The TRAX protein was established to contain bipartite nuclear targeting sequences in its N-terminal region, suggesting a possible role in the selective nuclear transport of Translin protein lacking any nuclear targeting motifs.
Insights
Researchers identified TRAX, a protein partner for Translin, a DNA-binding protein implicated in lymphoid malignancies. TRAX may facilitate Translin
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Translin is a DNA-binding protein that recognizes specific sequences at chromosomal translocation breakpoints.
- These translocations are frequently observed in lymphoid malignancies, suggesting Translin's role in these cancers.
Purpose of the Study:
- To investigate the functional significance of Translin at recombination hotspots.
- To identify other proteins that interact with Translin.
Main Methods:
- Yeast two-hybrid system to screen for protein interactions.
- Amino acid homology analysis to characterize identified partners.
Main Results:
- Identified a 33 kDa protein partner for Translin, named TRAX.
- TRAX shares extensive amino acid homology with Translin.
- TRAX possesses bipartite nuclear targeting sequences in its N-terminal region.
Conclusions:
- TRAX interacts with Translin.
- TRAX may be involved in the nuclear transport of Translin, which lacks its own nuclear targeting motifs.