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Ceramide induces apoptosis in PC12 cells

P J Hartfield1, G C Mayne, A W Murray

  • 1School of Biological Sciences, Flinders University of South Australia, Adelaide. P.Hartfield@flinders.edu.au

FEBS Letters
|January 20, 1997
PubMed
Summary

The lipid ceramide triggers programmed cell death (apoptosis) in PC12 cells. This cell death pathway involves specific enzymes and is influenced by ceramide concentration and cell density.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Ceramide is identified as a novel lipid second messenger.
  • Cellular responses to ceramide are not fully understood.

Purpose of the Study:

  • To investigate the role of ceramide in inducing apoptosis in PC12 cells.
  • To elucidate the mechanisms underlying ceramide-induced apoptosis.

Main Methods:

  • PC12 cells were treated with exogenous C2-ceramide.
  • Morphological analysis of nuclear changes and DNA fragmentation were performed.
  • Inhibition studies using Z-VAD.FMK were conducted.

Main Results:

  • Exogenous C2-ceramide induced apoptosis in a dose- and time-dependent manner.
  • Natural ceramide and C6-ceramide mimicked the apoptotic effect.
  • The response was specific to ceramide, as analogs did not induce apoptosis.
  • Apoptosis induction was dependent on cell plating density.
  • Z-VAD.FMK, an inhibitor of ICE-like proteases, blocked ceramide-induced nuclear changes.

Conclusions:

  • Ceramide is a potent inducer of apoptosis in PC12 cells.
  • Ceramide-induced apoptosis involves the activation of ICE-like proteases.
  • Cellular context, such as plating density, influences ceramide's apoptotic effect.

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