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The guide RNA database

A E Souza1, T Hermann, H U Göringer

  • 1Laboratorium für Molekulare Biologie, Genzentrum der LMU München am MPI für Biochemie, Am Klopferspitz 18, 82152 Martinsried, Germany.

Nucleic Acids Research
|January 1, 1997
PubMed
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This study compiles guide RNA (gRNA) sequences from protozoan parasites, offering valuable data on RNA editing. The database includes sequence information, gene localization, and structural details for enhanced research accessibility.

Area of Science:

  • Molecular Biology
  • Parasitology
  • Bioinformatics

Background:

  • RNA editing is a critical post-transcriptional modification in protozoan parasites.
  • Guide RNAs (gRNAs) are essential small RNA molecules that direct RNA editing.
  • Kinetoplastid organisms exhibit complex RNA editing mechanisms.

Purpose of the Study:

  • To create a comprehensive database of guide RNA (gRNA) sequences from kinetoplastid parasites.
  • To centralize information on gRNA primary sequences, gene localization, and experimental verification.
  • To provide structural insights into gRNAs, including secondary and 3D models.

Main Methods:

  • Compilation of published guide RNA sequences from eight kinetoplastid species.
  • Inclusion of data on gene localization and transcript verification.

Related Experiment Videos

  • Generation of secondary structures for select gRNAs and a computer-modeled 3D structure.

    • Main Results:

    • The gRNA database contains primary RNA sequences and associated metadata.
    • Detailed information on gene localization and experimental validation is provided.
    • Structural data, including secondary and 3D models, is available for specific gRNAs.

    Conclusions:

    • The gRNA database serves as a valuable resource for studying RNA editing in protozoan parasites.
    • Accessibility via the World Wide Web facilitates broader research use.
    • The integrated data supports further investigation into gRNA function and evolution.