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Isoguanine quartets formed by d(T4isoG4T4): tetraplex identification and stability
1Laboratorium für Organische und Bioorganische Chemie, Universität Osnabrück, Germany. fraseela@rz.uni-osnabrueck.de
Nucleic Acids Research
|December 15, 1996
Abstract:
The self-aggregation of the oligonucleotide d(T4isoG4T4) (1) is investigated. Based on ion exchange HPLC experiments and CD spectroscopy, a tetrameric structure is identified. This structure was formed in the presence of sodium ions and shows almost the same chromatographic mobility on ion exchange HPLC as d(T4G4T4) (2). The ratio of aggregate versus monomer is temperature dependent and the tetraplex of [d(T4isoG4T4)]4 is more stable than that of [d(T4G4T4)]4. A mixture of d(T4isoG4T4) and d(T4G4T4) forms mixed tetraplexes containing strands of d(T4isoG4T4) and d(T4G4T4).