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Systemically administered cycloheximide reduces inhibition in rat neocortical slice preparation
I Buchkremer-Ratzmann1, O W Witte
1Neurologische Klinik, Heinrich-Heine-Universität, Düsseldorf, Germany.
Brain Research
|December 16, 1996
Summary
Pharmacological inhibition of protein synthesis, using cycloheximide, reduces neocortical inhibition. This suggests that inhibiting protein synthesis may alter brain excitability after global cerebral ischemia.
Area of Science:
- Neuroscience
- Biochemistry
Background:
- Global cerebral ischemia causes lasting hyperexcitability and reduced protein synthesis in surrounding brain tissue.
- Altered brain excitability is a key feature following ischemic events.
Purpose of the Study:
- To investigate if pharmacological inhibition of protein synthesis directly impacts neocortical excitability.
- To understand the role of protein synthesis in regulating brain excitability.
Main Methods:
- Administration of the protein synthesis inhibitor cycloheximide via intraperitoneal injections.
- Assessment of neocortical inhibition at two hours post-treatment.
Main Results:
- A widespread reduction in neocortical inhibition was observed after cycloheximide administration.
- The effects were noted two hours after the final injection.
Conclusions:
- Pharmacological inhibition of protein synthesis can lead to decreased neocortical inhibition.
- This finding suggests that protein synthesis inhibition may be a contributing factor to altered brain excitability observed after ischemia.