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Detection of different metabolites in the rabbit lens by high resolution 1H NMR spectroscopy
A Midelfart1, A Dybdahl, S Gribbestad
1Department of Ophthalmology, Faculty of Medicine, Norwegian Institute of Technology, University of Trondheim, Norway.
Purpose:
To investigate the metabolic profile of the rabbit lens using high resolution 1H NMR spectroscopy including two-dimensional shift correlated (COSY) technique.
Methods:
Perchloric acid extracts of the rabbit lens were analysed with a Bruker AM-500 spectrometer and the metabolites were assigned in the spectra. Some of these were also quantified.
Results:
More than 20 metabolites were detected in the perchloric acid extract of a single lens, including amino acids, nucleotides and other related compounds. Of particular importance is the ability to detect and identify glutathione, myoinositol, scyllo-inositol and taurine.
Conclusions:
The study demonstrated the potential of 1H NMR spectroscopy for monitoring the metabolic profile of the lens in normal and pathologic conditions.