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Actin enhances the activation of human neutrophil NADPH oxidase in a cell-free system
T Morimatsu1, A Kawagoshi, K Yoshida
1Department of Applied Biochemistry, Faculty of Engineering, Ehime University, Matsuyama, Japan.
Biochemical and Biophysical Research Communications
|January 3, 1997
Abstract:
The cell-free activation of human neutrophil NADPH oxidase (02- generating enzyme) was enhanced by exogenously added G-actin (actin monomer). When cytosol, a constituent of the system, was pretreated with DNase I, which may bind to G-actin (endogenous) to block polymerization, the activation of NADPH oxidase was significantly suppressed. The activation was also impaired when cytosol G-actin was removed by DNase I-linked resin, being completely restored by the addition of G-actin. These results suggest a role of actin and its polymerization in the activation of NADPH oxidase of human neutrophils.