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Is wheat germ agglutinin (WGA) a glycosylated protein?
D G Kisiel1, A Gindzieński, K Zwierz
1Department of General and Organic Chemistry, Medical Academy of Białystok.
Roczniki Akademii Medycznej W Bialymstoku (1995)
|January 1, 1996
Abstract:
Lectin isolated from wheat germ was purified by affinity chromatography on ovogel column, recrystallization and gel filtration on Ultrogel AcA 44 column, eluted with borate buffer to eliminate accidental lectin-ligand interaction products. In 87-times purified WGA preparation fucose, xylose, mannose, galactose and glucose were found, amounting 1.5% of lectin mass.