Acrosin activity in pelleted frozen sperm does not correlate with in vitro fertilization of oocytes

Spermatozoa pelleted after swim-up were frozen and then analysed in batches for acrosin activity, using a spectrophotometric method, and expressed as microIU micrograms DNA-1. A total of 259 sperm samples were analysed and the acrosin activity compared with fertilization in vitro. Of these, 224 samples fertilized at least one oocyte and 35 samples failed to fertilize any oocyte. Analysis by Student's t-test indicated that there was a statistically significant difference (P = 0.02) in acrosin activity between the two groups. However, when samples that failed to fertilize were matched for concentration, motility and normal morphology with samples that fertilized, this significance was lost (P = 0.77). It is concluded that total acrosin in pelleted sperm frozen after regular swim-up, does not correlate with fertilizing ability of spermatozoa used for insemination.