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In situ detection of tandem DNA repeat length
R Yaar1, P Szafranski, C R Cantor
1Center for Advanced Biotechnology, Boston University, MA 02215, USA.
Genetic Analysis : Biomolecular Engineering
|November 1, 1996
Abstract:
A simple method for scoring short tandem DNA repeats is presented. An oligonucleotide target, containing tandem repeats embedded in a unique sequence, was hybridized to a set of complementary probes, containing tandem repeats known lengths. Single-stranded loops structures formed on duplexes containing a mismatched (different) number of tandem repeats. No loop structure formed on duplexes containing a matched (identical) number of tandem repeats. The matched and mismatched loop structures were enzymatically distinguished and differentially labeled by treatment with S1 nuclease and the Klenow fragment of DNA polymerase.