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Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80
G T Duncan1, K Balamurugan, B Budowle
1Department of Biology, Florida International University, Miami 33199, USA. gdunca01@servax.fiu.edu
Genetic Analysis : Biomolecular Engineering
|November 1, 1996
Abstract:
The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggest that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a BsoF I restriction site immediately adjacent to the repeat region.