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Synthesis and utilization of GDP-D-arabinopyranoside
S Park1, I Pastuszak, B J Mengeling
1Department of Biochemistry and Molecular Biology, University of Arkanasas for Medical Sciences, Little Rock 72211, USA.
Analytical Biochemistry
|January 15, 1997
Abstract:
We describe a procedure for the enzymatic synthesis of labeled or unlabeled GDP-D-arabinopyranoside. This method uses two enzymes purified from pig kidney: an L-fucokinase and a GDP-L-fucose pyrophosphorylase. The isolated GDP-D-[3H]arabinose served as a precursor for arabinose addition to lipophosphoglycan (LPG) of Leishmania major, using a parasite membrane fraction as the source of arabinosyltransferase. The procedures described provide a useful means for obtaining radiolabeled GDP-D-arabinopyranoside to study synthesis of D-arabinopyranoside-containing glycoconjugates.