Micellar electrokinetic capillary chromatographic analysis of platelet activating factor in human blood by indirect...

In this paper, 1-O-hexadecyl and 1-O-octadecyl-2-acetyl-sn-glycero-3- phosphorylcholine (C16-AGEPC and C18-AGEPC) in the platelet activating factor of human blood were analyzed by micellar electrokinetic capillary chromatography (MECC) with indirect ultraviolet absorption detection at 254 nm. The optimum running buffer for the separation contained 50 mmol/l sodium dodecy sulphate, 20 mmol/l potassium hydrogen phthalate, 10 mmol/l borax and 3 mol/l urea (pH 6.8). The separation was completed within 10 min. The detection limits of C16-AGEPC and C18-AGEPC were the same, i.e. 60 ng/ml (k = 3). The analytical precision (n = 6) was 2.8-3.0% and 1.4-1.7% for the determination (peak height mode) and for the measurement of the migration times, respectively. The application of this method to the clinical samples was demonstrated.