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Related Experiment Videos

Actin depolymerization is developmentally regulated in rat type II cells exposed to terbutaline

V Bhandari1, H Lu, J Pachter

  • 1Department of Pediatrics, University of Connecticut School of Medicine, Farmington 06030-2203, USA.

Pediatric Research
|February 1, 1997
PubMed
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The actin cytoskeleton in type II alveolar cells regulates surfactant secretion. Terbutaline triggers actin depolymerization, with timing differences between adult and fetal cells, suggesting developmental regulation of this secretory pathway.

Area of Science:

  • Cell Biology
  • Pulmonary Medicine
  • Developmental Biology

Background:

  • Type II alveolar epithelial cells synthesize and secrete pulmonary surfactant.
  • Terbutaline is known to enhance phospholipid release from these cells.
  • The role of the actin cytoskeleton in regulating this secretory activity is not fully understood.

Purpose of the Study:

  • To investigate the developmental regulation of actin subfractions in type II cells during secretory activity.
  • To examine the effects of terbutaline on the actin network in adult and fetal type II cells.
  • To determine if actin depolymerization is a key mechanism in surfactant secretion.

Main Methods:

  • Type II cells from adult and fetal rat lungs were cultured and treated with terbutaline.

Related Experiment Videos

  • Globular (G-actin) and filamentous (F-actin) fractions were isolated and analyzed.
  • Western blotting and densitometry were used to quantify actin subfractions.

    • Main Results:

    • Terbutaline caused rapid F-actin depolymerization in adult cells within 1 minute, returning to baseline by 60 minutes.
    • In fetal cells, F-actin depolymerization peaked at 60 minutes after terbutaline exposure.
    • Actin depolymerization was dose-dependent and also induced by phorbol ester.

    Conclusions:

    • Actin microfilament depolymerization appears to regulate the transport and exocytosis of lamellar bodies in type II cells.
    • Adult type II cells may possess an early actin-dependent and a late actin-independent secretory mechanism.
    • The timing of the actin-dependent pathway is developmentally regulated, explaining observed differences in surfactant secretion.