Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mammalian cell DNA replication

R J Hickey1, L H Malkas

  • 1University of Maryland, School of Medicine, Baltimore 21201, USA.

Critical Reviews in Eukaryotic Gene Expression
|January 1, 1997
PubMed
Summary

Understanding mammalian DNA replication is crucial. This review details key proteins, their functions, and how they form replication factories for efficient DNA synthesis and cell cycle regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective cell adhesion on femtosecond laser-microstructured polydimethylsiloxane.

Biomedical materials (Bristol, England)·2016
Same author

Ascosin, an antifugal antibiotic produced by a streptomycete.

Antibiotics & chemotherapy (Northfield, Ill.)·2014
Same author

Effects of tampons and menses on the composition and diversity of vaginal microbial communities over time.

BJOG : an international journal of obstetrics and gynaecology·2013
Same author

Phosphorylation: the molecular switch of double-strand break repair.

International journal of proteomics·2011
Same author

Cancer-associated alteration of pericentromeric heterochromatin may contribute to chromosome instability.

Oncogene·2011
Same author

Precipitation of riboflavin from aqueous solution by bacteriological reduction.

Archives of biochemistry·2010

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Mammalian DNA synthesis regulation mechanisms remain unclear.
  • In vitro systems like the simian virus 40 (SV40)-based cell-free system have identified key DNA replication enzymes and proteins.
  • The functional organization of these proteins into efficient DNA replication machinery is not well-defined.

Purpose of the Study:

  • To review identified proteins involved in mammalian DNA replication.
  • To discuss proposed mechanisms of action for these proteins.
  • To explore the organization of proteins into multiprotein complexes or 'replication factories' for chromosomal DNA synthesis.

Main Methods:

  • Literature review of studies on mammalian DNA replication.
  • Analysis of in vitro DNA replication systems, including SV40-based systems.
  • Discussion of proposed protein functions and organizational models.

Main Results:

  • Several mammalian enzymes and proteins essential for DNA synthesis have been identified.
  • Proposed mechanisms of action for these proteins are described.
  • Models for protein organization into multiprotein complexes (replication factories) are discussed.

Conclusions:

  • Further research is needed to fully elucidate the precise mechanisms regulating mammalian DNA synthesis.
  • Understanding protein organization is key to understanding efficient chromosomal DNA synthesis.
  • The cell cycle regulation and replication origins of mammalian DNA synthesis are critical areas of ongoing study.

Related Experiment Videos