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Microwave fixation method for cytochemistry. For conventional electron microscopy, enzymo-immunocytochemistry,
1Medical Research Institute, Tokyo Medical and Dental University, Japan.
European Journal of Morphology
|December 1, 1996
Abstract:
More than a decade ago, we introduced a microprocessed household microwave oven for microwave-stimulated fixation. In this paper we present an overview of our experience. We have introduced the use of tannic acid (TA, 0.1%), which is very effective for fixing soluble peptides or proteins, except in the case of enzymo-cytochemistry. In the latter case, a combination of TA with aldehyde in the microwave-fixation step brings perfect fixation, but inactivation of the enzymes and their enzymatic activities.