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Related Experiment Videos

Pulmonary microvascular reflection coefficients estimated with modified lymphatic washdown technique

R E Drake1, S Dhother, J C Gabel

  • 1Department of Anesthesiology, University of Texas Medical School, Houston 77030, USA.

The American Journal of Physiology
|January 1, 1997
PubMed
Summary

This study refined the lymphatic protein washdown technique to better estimate the pulmonary microvascular reflection coefficient (sigma d). New methods yielded a significantly higher sigma d estimate, improving accuracy in lung research.

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Area of Science:

  • Pulmonary physiology
  • Cardiovascular research
  • Microvascular function

Background:

  • The lymphatic protein washdown technique is commonly used to estimate the pulmonary microvascular reflection coefficient to protein (sigma d).
  • Previous methods may underestimate sigma d due to protein contamination from lung tissue and the opposing effect of plasma protein osmotic pressure (IIc).

Purpose of the Study:

  • To develop a more accurate method for estimating sigma d by minimizing confounding factors.
  • To re-evaluate sigma d in anesthetized sheep using an improved technique.

Main Methods:

  • Reduced IIc to 5.6 +/- 1.1 mmHg and increased pulmonary microvascular pressure to 22 +/- 3 mmHg in five anesthetized sheep.
  • Utilized Evans blue dye to tag plasma protein for estimating sigma d from lymph and plasma concentrations.

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  • Avoided interference from tissue protein by not tagging it.

    • Main Results:

    • The estimated sigma d was 0.79 +/- 0.08.
    • This estimate is significantly higher than previously reported values in anesthetized animals.

    Conclusions:

    • The refined technique provides a more accurate estimation of sigma d.
    • Higher sigma d values suggest tighter pulmonary microvascular barrier integrity than previously thought.