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Updated: Oct 12, 2026

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method
Published on: July 31, 2007
Transformation of Escherichia coli cells by pH 2.3 plasmid DNA treated with psoralens plus near-UV light
R Repanovici1, A Plesa, G Anton
1Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
Abstract:
The effect of two psoralens, 8-methoxypsoralen (8MOP) and angelicin (ANG), plus near-UV (PUVA) on the transformation capacity of pH 2.3 plasmid DNA on Escherichia coli was studied. Under identical experimental conditions the 8MOP linking to plasmid DNA drastically decreased its transformation capacity compared with the ANG linking. In the case of 8MOP, the decrease depends on the UV dose, as well as on the molar ratios of psoralen and DNA nucleotides. When the effect of short-wavelength UV (UVB) was tested, the higher the molar ratios, the more the combined effects of PUVA and UVB were negative.
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