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Related Experiment Video

Updated: May 12, 2026

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: November 1, 2012

Peripheral blood stem cells for autografting

E J Shpall¹, P J Cagnoni, S I Bearman

¹University of Colorado Bone Marrow Transplant Program, University of Colorado, Health Sciences Center, Denver 80262, USA.

Annual Review of Medicine

|January 1, 1997

Summary

High-dose therapy using hematopoietic progenitor cells effectively treats high-risk cancers. Peripheral blood stem cells are replacing marrow for faster recovery, improving treatment safety and cost-effectiveness.

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Area of Science:

Hematology
Oncology
Stem Cell Transplantation

Background:

High-dose therapy with autologous hematopoietic progenitor cell support is a recognized treatment for various high-risk malignancies.
Accelerating marrow recovery through hematopoietic cell support enhances the safety and cost-effectiveness of high-dose regimens.
Peripheral blood progenitor cells are emerging as a primary source for hematopoietic support, potentially replacing bone marrow.

Purpose of the Study:

To review the techniques involved in peripheral blood progenitor cell collection.
To discuss methods for mobilizing progenitor cells into the peripheral blood.
To cover purification techniques for tumor removal and ex vivo expansion methods.

Main Methods:

Review of established and emerging techniques for peripheral blood progenitor cell collection.
Description of mobilization strategies to enhance circulating progenitor cell yields.
Explanation of purification methods, including tumor purging, and ex vivo expansion protocols.

Main Results:

Peripheral blood progenitor cells are increasingly utilized for hematopoietic support.
Mobilization and collection techniques are crucial for successful stem cell transplantation.
Purification and expansion offer potential for improved therapeutic outcomes.

Conclusions:

High-dose therapy supported by hematopoietic progenitor cells is an effective oncologic treatment.
Peripheral blood progenitor cells are becoming the standard for hematopoietic support.
Advancements in collection, mobilization, purification, and expansion are key to optimizing this therapeutic approach.