Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural rules and conformational compensations in the tRNA L-form

S Steinberg1, F Leclerc, R Cedergren

  • 1Departement de Biochimie, Universite de Montreal, Quebec, Canada.

Journal of Molecular Biology
|February 21, 1997
PubMed
Summary

Mitochondrial tRNAs (mtRNAs) were modeled using a new structural criterion. Compensatory mechanisms in mtRNA structure allow for proper three-dimensional folding, essential for function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rare and Common Variants Conferring Risk of Tooth Agenesis.

Journal of dental research·2018
Same author

15q11.2 CNV affects cognitive, structural and functional correlates of dyslexia and dyscalculia.

Translational psychiatry·2017
Same author

A Genomics Approach to Mitochondrial Evolution.

The Biological bulletin·2017
Same author

A rare missense mutation in CHRNA4 associates with smoking behavior and its consequences.

Molecular psychiatry·2016
Same author

Genome-wide meta-analysis reveals common splice site acceptor variant in CHRNA4 associated with nicotine dependence.

Translational psychiatry·2015
Same author

[Propranolol and lactatemia during hypovolemic shock: a case report].

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2014

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Genetics

Background:

  • Mitochondrial tRNAs (mtRNAs) exhibit diverse secondary structures.
  • Understanding mtRNA three-dimensional conformation is crucial for deciphering their function.

Purpose of the Study:

  • To develop and apply a new criterion for modeling the three-dimensional structure of mtRNAs.
  • To investigate structural compensations in mtRNAs that deviate from established models.

Main Methods:

  • Analysis of known cytosolic tRNA structures to derive a novel modeling criterion.
  • Three-dimensional modeling of five distinct mtRNA secondary structure types.
  • Investigating structural compensations, including nucleotide intercalation and conformational flexibility.

Related Experiment Videos

Main Results:

  • A new criterion based on nucleotide stacking (59 of T-loop onto Domain 1) was established.
  • Deviations in mtRNA structure were successfully modeled using compensatory mechanisms.
  • Intercalation of unpaired nucleotides and flexible linker regions were identified as key compensations.

Conclusions:

  • The stacking of nucleotide 59 onto Domain 1 is essential for the three-dimensional L-shape of tRNAs.
  • Structural compensations in mtRNAs ensure proper folding despite sequence variations.
  • This study provides insights into the structural adaptability of mitochondrial tRNAs.