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Related Experiment Videos

Do DNA vaccines induce autoimmune disease?

G Mor1, M Singla, A D Steinberg

  • 1Section of Retroviral Immunology, Food and Drug Administration, Bethesda, MD 20892, USA.

Human Gene Therapy
|February 10, 1997
PubMed
Summary

This study investigated DNA vaccines and autoimmunity. Results show DNA vaccines did not trigger anti-DNA or anti-muscle autoantibodies, nor did they accelerate autoimmune disease in lupus-prone mice.

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Area of Science:

  • Immunology
  • Vaccinology
  • Autoimmunity

Background:

  • DNA vaccines are a novel vaccine platform.
  • Concerns exist regarding their potential to induce autoimmunity.

Purpose of the Study:

  • To determine if plasmid DNA vaccines induce autoantibody production.
  • To assess the impact of DNA vaccines on autoimmune disease development.

Main Methods:

  • BALB/c mice and lupus-prone (NZB x NZW)F1 mice were immunized with DNA plasmids.
  • Quantification of B cells secreting anti-DNA autoantibodies.
  • Monitoring for anti-muscle cell autoantibodies and clinical signs of autoimmune disease.

Main Results:

  • A significant increase in B cells producing immunoglobulin G (IgG) anti-DNA autoantibodies was observed post-vaccination.

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  • This increase correlated with transient serum anti-DNA autoantibody titers but not disease.
  • No evidence of anti-muscle cell autoantibodies or myositis was found.
  • DNA vaccination did not affect the onset or progression of autoimmune disease in lupus-prone mice.

    • Conclusions:

    • Plasmid DNA vaccines do not appear to initiate or accelerate systemic autoimmunity.
    • The observed autoantibody production is transient and not pathogenic.
    • DNA vaccines are unlikely to pose a risk for inducing or worsening autoimmune conditions.