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Flow cytometric platelet enumeration utilizing monoclonal antibody CD42a
C Tanaka1, T Ishii, K Fujimoto
1Scientific Division, R&D, TOA Medical Electronics Co., Ltd, Kobe, Japan.
Clinical and Laboratory Haematology
|December 1, 1996
Summary
A flow cytometric method using CD42a antibody offers a reliable reference for platelet counts. This technique accurately calibrates platelet enumeration by automated blood cell analyzers.
Area of Science:
- Hematology
- Immunology
- Analytical Chemistry
Background:
- Accurate platelet enumeration is critical for diagnosing and managing various hematological conditions.
- Current automated blood cell analyzers require reliable reference methods for calibration and quality control.
Purpose of the Study:
- To evaluate a flow cytometric method utilizing the monoclonal antibody CD42a as a reference standard for platelet counting.
- To assess the correlation between this flow cytometric method and existing automated blood count analyzers.
Main Methods:
- Peripheral blood samples from 25 healthy individuals were analyzed.
- Flow cytometry was performed using the FACScan system with monoclonal antibody CD42a.
- Results were compared against multiple automated cell counters (TOA Medical Electronics).
Main Results:
- The flow cytometric method demonstrated significant correlations with all tested cell counters (r values ranging from 0.949 to 0.960, P < 0.0001).
- High correlation coefficients indicate excellent agreement between the reference method and commercial analyzers.
Conclusions:
- The flow cytometric method with CD42a antibody is a validated and accurate reference method for platelet enumeration.
- This method can be effectively employed to verify and calibrate platelet counts performed by automated blood cell analyzers.