In vitro calcium phosphate formation on a natural composite material, bamboo

A natural self-reinforced composite material, bamboo, is studied for the first time as a biomedical material. Its anatomical structure was investigated and its mechanical properties were measured and compared with those of some common bone-bonding or bone-repairing biomaterials. It is found that, among all kinds of biomaterials, bamboo has the closest modulus of elasticity to human long bone. The cytotoxicity of bamboo was tested using the agar overlay method before and after heat or chemical treatments. The results reveal that ethanol, methanol and toluene can remove toxic leachable components from bamboo to some extent through extraction. After grafting a polymer whose molecule includes poly(ethylene glycol), alpha,omega-di(aminopropyl)poly(ethylene glycol) 800 on bamboo, bamboo has the ability to form a calcium phosphate coating after being immersed in calcification solution (simulated body fluid and accelerated calcification solution). The characteristics and the morphology of the mineral formed on bamboo were studied by infrared spectroscopy and scanning electron microscopy.