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Related Experiment Videos

Hemopoietic stem cells bear sperm-associated antigens

R L Krogsrud, G B Price

    Experimental Hematology
    |August 1, 1979
    PubMed
    Summary

    Researchers identified unique surface antigens on murine hemopoietic stem cells. These stem cells show cross-reactivity with sperm, brain, and embryonic antigens, aiding in their isolation.

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    Area of Science:

    • Immunology
    • Hematology
    • Cell Biology

    Background:

    • Murine hemopoietic stem cells (HSCs) are crucial for blood formation.
    • Identifying unique HSC surface markers is essential for their isolation and study.
    • Previous research suggested potential cross-reactivity of HSC antigens with other cell types.

    Purpose of the Study:

    • To enrich populations of murine hemopoietic stem cells using fluorescence-activated cell sorting (FACS).
    • To investigate the expression of specific surface antigens on murine HSCs.
    • To test the hypothesis that HSCs share surface antigens with sperm, brain, and embryonic cells.

    Main Methods:

    • Utilized fluorescence-activated cell sorting (FACS) for cell separation.
    • Labeled mouse bone marrow cells with various antisera, including anti-human brain, anti-human sperm, anti-mouse F9, and anti-mouse Ia.
    • Quantified enrichment of stem cells based on fluorescence signals.

    Main Results:

    • Successfully enriched murine hemopoietic stem cell populations using FACS.
    • Demonstrated significant positive enrichment with rabbit anti-human brain, human anti-human sperm, and mouse anti-mouse F9 sera.
    • Showed no significant enrichment using mouse anti-mouse Ia serum.

    Conclusions:

    • Murine hemopoietic stem cells possess unique surface antigens.
    • These HSC surface antigens exhibit cross-reactivity with antigens found on sperm, brain, and embryonic cells.
    • The identified antigens can be utilized for the specific isolation of functional murine HSCs.

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