Related Experiment Video
Updated: Aug 15, 2026

Corneal Donor Tissue Preparation for Endothelial Keratoplasty
Published on: June 12, 2012
Acanthamoeba infection after radial keratotomy
R F Friedman1, T C Wolf, J Chodosh
1Department of Ophthalmology, Dean A. McGee Eye Institute, USA.
Purpose:
To describe a case of Acanthamoeba infection of the cornea after radial and astigmatic keratotomy.
Methods:
A 29-year-old man developed ulcerative keratitis in the right eye 6 weeks after uncomplicated radial and astigmatic keratotomy.
Results:
Three sets of corneal cultures for bacteria and fungi were negative. Culture on non-nutrient agar grew Acanthamoeba organisms. Clinical improvement occurred after topical antiamebic therapy was instituted.
Conclusions:
Incisional keratotomy may predispose the cornea to delayed-onset infectious keratitis. Acanthamoeba should be considered as a possible cause of infection and should be cultured for in refractory cases.

