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[The lysozyme-synthetizing activity of enterococci and lactobacilli]
Mikrobiolohichnyi Zhurnal (Kiev, Ukraine : 1993)
|November 1, 1996
Summary
Lactic acid bacteria, particularly Enterococcus species from specific sources, exhibit significant lysozyme production. This enzyme synthesis is a key factor in their biological activity and can aid in identifying certain lactobacilli.
Area of Science:
- Microbiology
- Enzymology
- Biochemistry
Background:
- Lactic acid bacteria (LAB) are known for their diverse biological activities.
- Lysozyme, an antimicrobial enzyme, plays a role in host defense and microbial interactions.
- Understanding lysozyme production by LAB can reveal insights into their functional roles.
Purpose of the Study:
- To investigate the lysozyme-synthesizing activity across a broad range of lactic acid bacteria.
- To determine the influence of isolation source on lysozyme production in LAB.
- To evaluate the potential diagnostic value of lysozyme production for bacterial identification.
Main Methods:
- Culturing of lactic acid bacteria from various natural substrates.
- Assaying lysozyme production quantitatively or qualitatively.
- Comparative analysis of lysozyme synthesis based on bacterial genus, species, and isolation origin.
Main Results:
- Lysozyme production varied significantly among different lactic acid bacteria strains and sources.
- Enterococcus species isolated from the digestive tracts of long-living humans, fish, geese, and ducks showed high lysozyme activity.
- Lysozyme-synthesizing capacity in lactobacilli was strain-dependent and linked to species and group classification, suggesting diagnostic utility for Betabacterium subgenus.
Conclusions:
- The source of isolation is a critical factor influencing lysozyme production in lactic acid bacteria.
- Enterococcus and specific Lactobacillus strains are significant producers of lysozyme.
- Lysozyme production serves as a valuable characteristic for the identification and classification of lactic acid bacteria, particularly within the Betabacterium subgenus.