Related Experiment Videos
Reproducibility of human cardiac 31P-NMR spectroscopy
H J Lamb1, J Doornbos, J A den Hollander
1Department of Diagnostic Radiology and Nuclear Medicine, Leiden University Medical Centre, The Netherlands.
NMR in Biomedicine
|August 1, 1996
Summary
Reproducibility of cardiac phosphocreatine to adenosine triphosphate ratio (PCr/ATP) was assessed using phosphorus-31 (31P) NMR. Three-dimensional localization is crucial for accurate PCr/ATP measurements in the human left ventricle.
Area of Science:
- Cardiovascular research
- Medical imaging
- Biophysics
Background:
- The phosphocreatine to adenosine triphosphate ratio (PCr/ATP) is a key indicator of cardiac energy metabolism.
- Accurate measurement of PCr/ATP using phosphorus-31 (31P) Nuclear Magnetic Resonance (NMR) is essential for diagnosing heart disease.
- Previous studies have faced challenges in achieving reproducible PCr/ATP measurements due to signal contamination and localization issues.
Purpose of the Study:
- To assess the reproducibility of cardiac PCr/ATP measurements in the human left ventricle.
- To compare the effectiveness of three different localization techniques in cardiac 31P-NMR spectroscopy.
- To determine the reliability of consecutive cardiac 31P-NMR spectral acquisitions for metabolic evaluation.
Main Methods:
- Cardiac 31P-NMR spectra were acquired from 16 healthy subjects (n=68) using three-dimensional (3D) image selected in vivo spectroscopy (ISIS), 1D spectroscopic imaging (SI), or a combination (ISIS + SI).
- The PCr/ATP ratio was calculated for each localization technique.
- Intra- and inter-examination differences in PCr/ATP were analyzed to assess reproducibility.
Main Results:
- The SI technique yielded significantly lower PCr/ATP values (0.98 +/- 0.20) compared to ISIS + SI (1.41 +/- 0.20) and ISIS (1.31 +/- 0.19), indicating signal contamination from surrounding tissues like the liver.
- Three-dimensional localization (ISIS and ISIS + SI) provided more accurate and reliable PCr/ATP measurements.
- Absolute intra-examination differences in PCr/ATP were significantly smaller than inter-examination differences across all techniques, suggesting good short-term reproducibility.
Conclusions:
- Three-dimensional localization techniques are essential for accurate cardiac PCr/ATP quantification using 31P-NMR, preventing liver signal contamination.
- Consecutive acquisition of cardiac 31P-NMR spectra within a single examination is a reliable method for metabolic evaluation of heart disease.
- This study validates the use of cardiac 31P-NMR spectroscopy with appropriate localization for assessing myocardial energy status.