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Partial structure, chromosome localization, and expression of the mouse Icln gene
K Wickman1, M F Seldin, M R James
1Children's Hospital Medical Center, Harvard Medical School, Enders Building, Room 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA.
Genomics
|March 15, 1997
Summary
Researchers identified the mouse Icln gene, a regulator of chloride channels, and mapped its location on human and mouse chromosomes. Abundant ICln mRNA expression supports its role in regulating ubiquitous chloride channels.
Area of Science:
- Molecular biology
- Genetics
- Physiology
Background:
- The molecular basis of volume-regulated ion channels remains largely unknown.
- The nucleotide-sensitive chloride conductance regulator, ICln, has been implicated in volume-regulated chloride channel function.
Purpose of the Study:
- To clone a fragment of the mouse Icln gene.
- To identify Icln pseudogenes.
- To map the functional Icln gene in humans and mice.
Main Methods:
- Gene cloning techniques.
- Pseudogene identification.
- Chromosomal mapping using genetic markers.
- mRNA expression analysis via tissue distribution studies.
Main Results:
- A fragment of the mouse Icln gene was successfully cloned.
- Several putative Icln pseudogenes were identified.
- The functional Icln gene was mapped to human chromosome 11q13.5-q14 and mouse chromosome 7.
- ICln mRNA demonstrated widespread and consistent expression across all examined mouse tissues and developmental stages.
Conclusions:
- The cloning and mapping of the mouse Icln gene provide a foundation for further functional studies.
- The ubiquitous expression pattern of ICln mRNA supports its proposed role as a regulator of volume-regulated chloride channels.