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Protein inheritance: lambda plasmid replication perpetuated by the heritable replication complex

A Wegrzyn1, G Wegrzyn, A Herman

  • 1Polish Academy of Sciences, Laboratory of Molecular Biology affiliated to the University of Gdańsk.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|November 1, 1996
PubMed
Summary
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The lambda O initiator protein complex, assembled before a temperature shift, is inherited across cell generations, driving plasmid replication. This protein inheritance phenomenon bypasses the need for new complex assembly.

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Bacterial plasmid replication relies on specific protein-DNA interactions at the origin of replication.
  • The Escherichia coli phage lambda replication system involves lambda O and lambda P proteins.
  • A mutation (ts1) in lambda P protein impairs replication complex assembly at elevated temperatures.

Purpose of the Study:

  • To investigate the fate and function of a pre-assembled lambda replication complex under restrictive temperature conditions.
  • To determine if the replication complex can be inherited across cell divisions.

Main Methods:

  • Utilizing a lambda crotsPts1 plasmid in Escherichia coli.
  • Performing temperature upshift experiments from 30°C to 43°C.
  • Assessing replication inheritance and dependence on specific genes (lambda O, dnaK, dnaJ, grpE).

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Main Results:

  • The replication complex assembled at 30°C remains functional after a temperature upshift to 43°C.
  • This pre-assembled complex is inherited through successive replication rounds for over 30 generations.
  • Replication driven by the inherited complex is resistant to chloramphenicol and sensitive to rifampicin, depending on lambda O and host chaperone functions.

Conclusions:

  • The lambda O-containing replication complex is inherited alongside DNA, retaining the ability to initiate replication.
  • This inheritance of a functional replication complex represents a novel form of protein inheritance.