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Determination of lysergide in urine by high-performance liquid chromatography combined with electrospray ionisation
S A White1, T Catterick, M E Harrison
1Laboratory of the Government Chemist, Teddington, Middlesex, UK.
Journal of Chromatography. B, Biomedical Sciences and Applications
|February 21, 1997
Abstract:
A quantitative method which avoids derivatisation is described for the determination of lysergide (LSD) levels in urine. Sample preparation included addition of methysergide as an internal standard followed by solid-phase extraction. LSD was analysed on a system consisting of a C18 stationary phase and a mobile phase of 0.1 M acetate buffer pH 8.0-acetonitrile-triethylamine (75:25:0.25, v/v). LSD was detected by electrospray ionisation mass spectrometry with selected ion monitoring. The quantification limit was 0.5 ng/ml and the method was linear up to 10 ng/ml of LSD in urine.