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Assay for the determination of urinary 8-hydroxy-2'-deoxyguanosine by high-performance liquid chromatography with
D Germadnik1, A Pilger, H W Rüdiger
1Department of Occupational Medicine, University of Vienna/Vienna General Hospital, Austria.
Journal of Chromatography. B, Biomedical Sciences and Applications
|February 21, 1997
Abstract:
A high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2'-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2'-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2'-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage.