The discrimination and durability of an ethanol cue in young and mid-aged female mice

Young adult (6 months) and mid-aged (12 months) C57BL/6 mice both learned to discriminate ethanol (ETOH, 1.0 g/kg) although criterion performance occurred later for mid-aged mice. ETOH discrimination improved with increasing dose (0.25-1.0 g/kg) and the dose-response function was unaffected by age. The ETOH cue had declined by 40 min postinjection for young mice not unlike a previous report for young rats. In contrast, the ETOH cue remained discriminable at 40 min for mid-aged mice, an effect perhaps due to their slower rate of ETOH metabolism and accountable for the previously reported reduction in ethanol consumption by mid-aged mice. Retention tests and reacquisition training both indicated that the ETOH cue can be retained by both age groups for at least 60 days without discrimination training or food deprivation. The present study suggests that the ethanol discriminative cue in mid-aged mice does not differ from that in young adult mice in potency but is more long lasting, the latter perhaps being related to their reduced ethanol consumption. Of significance from a therapeutic perspective, is that the ETOH cue remained discriminable for 2 months in both age groups (i.e., approximately 1/12 of their total life span).