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Updated: Jul 30, 2026

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
Published on: September 7, 2018
Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis
S E Miller1, R M Levenson, C Aldridge
1Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA. saram@acpub.duke.edu
Abstract:
A correlative microscopy method for the ultrastructural analysis of focal viral tissue infections is presented. Using a confocal scanning laser microscope, foci of infection are identified in tissue sections prior to embedment; a variety of techniques can be employed for viral detection, including staining with standard histochemical reagents and fluorescently labeled antibodies. Areas of infection identified using confocal microscopy are excised from the tissue sections, embedded, and examined by transmission electron microscopy. Applications of this technique in both diagnostic and basic research settings are described.
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