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Reduction of adhesion formation with hyaluronic acid after peritoneal surgery in rabbits
K E Rodgers1, D B Johns, W Girgis
1Livingston Reproductive Biology Laboratory, University of Southern California School of Medicine, Los Angeles 90033, USA.
Objective:
To examine the effect of hyaluronic acid, a high-molecular-weight glucosaminoglycan found in the extracellular matrix, on the formation of adhesions, a major source of postoperative complications.
Design:
The ability of hyaluronic acid to reduce adhesion formation was evaluated using a standardized rabbit model. The material was administered i.p. at the end of surgery.
Setting:
University laboratory.
Animal(S):
New Zealand White female rabbits.
Intervention(S):
Intraperitoneal administration of various formulations of hyaluronic acid at the end of surgery.
Main Outcome Measure(S):
One week after surgery, a second laparotomy was performed and the extent of adhesion formation was determined.
Result(S):
Five separate molecular weight ranges of hyaluronic acid representing eight viscosities between 1,000 and 12,000 centipoise (CPS) were shown to reduce adhesion formation in this model. All volumes, 1 to 30 mL, of hyaluronic acid tested reduced adhesion formation. In addition, the low-viscosity, low-molecular-weight hyaluronic acid significantly reduced adhesion formation when added to the trauma site or when injected at a site remote from the trauma area.
Conclusion(S):
This study showed that hyaluronic acid administered at the end of surgery reduced adhesion formation.