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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
Published on: December 5, 2011
Research on fermentation scale-up based on the OUR obtained from a shake flask
Chinese Journal of Biotechnology
|January 1, 1996
Summary
This study determined oxygen transfer parameters for fermentation scale-up. While oxygen uptake rate and biomass were consistent, volumetric oxygen transfer coefficient and cell density varied between shake flasks and fermentors.
Area of Science:
- Biochemical Engineering
- Bioprocess Engineering
- Fermentation Technology
Background:
- Accurate determination of oxygen transfer parameters is crucial for successful fermentation scale-up.
- Shake flasks are commonly used for initial fermentation studies, but their scalability to larger fermentors can be challenging.
- Understanding oxygen mass transfer limitations is essential for optimizing microbial growth and product formation.
Purpose of the Study:
- To determine oxygen permeability (Pm) of gauze layers using sulfite oxidation.
- To measure oxygen uptake rate (OUR) and volumetric oxygen transfer coefficient (KLa) during fermentation.
- To evaluate the feasibility of fermentor scale-up based on shake flask data.
Main Methods:
- Sulfite oxidation method for oxygen permeability determination.
- Measurement of gas-phase and liquid-phase oxygen concentrations during fermentation.
- Utilizing mass spectrometry and manual sampling for scale-up analysis in a 2.5 L fermentor.
- Determining oxygen uptake rate (OUR) and volumetric oxygen transfer coefficient (KLa).
Main Results:
- Oxygen permeability (Pm) of gauze layers was determined.
- Oxygen uptake rate (OUR) and biomass yield were comparable between shake flask and fermentor scales.
- Volumetric oxygen transfer coefficient (KLa) and cell density (CL) showed significant differences between scales.
Conclusions:
- Fermentation scale-up based on maximum oxygen uptake rate (OURmax) from shake flasks can yield similar biomass.
- Significant discrepancies in volumetric oxygen transfer coefficient (KLa) and cell density (CL) necessitate careful consideration during scale-up.
- Further investigation is needed to reconcile differences in oxygen transfer dynamics between shake flask and fermentor systems.
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