[Polylysogeny in Bacillus thuringiensis var. Galleriae cultures]

The culture of Bacillus thuringiensis var. galleriae was shown to be polylysogenic. As has been found earlier, the culture has a phage with the original structure of particles whose tail possesses a special substructure called a "collar". The phage was described in detail as a phage of Bac. thuringiensis 1-97 with a "collar". Further studies of the culture of Bac. thuringiensis var. galleriae 1-97 has shown that it also contains another phage which differs sharply from the first one in the morphology of particles, the spectrum of lytic action, and other properties. Electron microscopy of the cultural fluid of the Bac. thuringiensis var. galleriae cultures has revealed in them the presence of different amounts of the particles of both morphological types found in the strain 1-97. These data suggest that the cultures of Bac. thuringiensis var. galleriae are polylysogenic. The methods for the isolation of the new phage are described.