[Production of isolated colonies in Blakeslea trispora]

The effect of several compounds on the growth of Blakeslea trispora producing beta-carotene was studied on solid nutrient media. The paramorphous action was manifested by beta-ionone in the presence of sunflower oil. L-sorbose and beetroot pectin. Continuous growth of the fungus was changed into colonial growth by using a bilayered wort-agar medium. Mutants of Blakeslea trispora having compact type of growth have been isolated.