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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Mac-1 (CD11b/CD18) is an oligodeoxynucleotide-binding protein

L Benimetskaya1, J D Loike, Z Khaled

  • 1Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA.

Nature Medicine
|April 1, 1997
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Oligodeoxynucleotides bind to Mac-1 (CD11b/CD18) on immune cells, mediating their internalization. This interaction impacts immune cell function, suggesting Mac-1 as a receptor for these molecules.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Mac-1 (CD11b/CD18) is a key integrin on myeloid cells involved in immune responses.
  • Oligodeoxynucleotides (ODNs) have diverse therapeutic applications, but their cellular interactions require further elucidation.

Purpose of the Study:

  • To investigate the interaction between oligodeoxynucleotides and the Mac-1 integrin.
  • To determine the functional consequences of ODN binding to Mac-1 on immune cells.

Main Methods:

  • Studied binding of phosphodiester and phosphorothioate ODNs to Mac-1 on polymorphonuclear leukocytes (PMNs).
  • Utilized flow cytometry, competitive binding assays with fibrinogen, and anti-Mac-1 antibodies.
  • Assessed ODN internalization and functional effects on PMN migration and reactive oxygen species production.

Main Results:

  • Oligodeoxynucleotides bind to both alpha M and beta 2 subunits of Mac-1.
  • Fibrinogen competed with ODN binding, and anti-Mac-1 antibodies inhibited it.
  • Increased Mac-1 expression enhanced ODN binding and internalization, affecting PMN migration and reactive oxygen species production.

Conclusions:

  • Mac-1 serves as a cell-surface receptor for oligodeoxynucleotides.
  • ODN binding to Mac-1 mediates internalization and influences immune cell functions.
  • This interaction has significant functional implications for ODN-based therapies.