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Related Experiment Videos

Impinger sampling coupled to high-performance liquid chromatography by a modified autoinjector interface

H Schlitt1

  • 1Joint Research Centre of the European Union, Environment Institute, Ispra/VA, Italy.

Journal of Chromatography. A
|February 21, 1997
PubMed
Summary

This study introduces an automated device for air pollutant sampling and enrichment using a glass impinger coupled with high-performance liquid chromatography (HPLC). The system enables on-line analysis of airborne aldehydes and ketones at ng/l concentrations.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Chromatography

Background:

  • Air pollution monitoring requires efficient sampling and analysis techniques.
  • Traditional methods for analyzing airborne pollutants can be time-consuming and labor-intensive.
  • Automating the process of pollutant collection and preparation for analysis is crucial for improving efficiency.

Purpose of the Study:

  • To develop and validate a novel automated device for on-line sampling and enrichment of air pollutants.
  • To integrate a glass impinger with a high-performance liquid chromatography (HPLC) system for seamless analysis.
  • To determine airborne concentrations of aldehydes and ketones in an urban environment.

Main Methods:

  • A modified autosampler was used to automate the sampling and enrichment of air pollutants using a glass impinger.

Related Experiment Videos

  • Air was bubbled through a reaction solution containing 2,4-dinitrophenylhydrazine (DNPH) and phosphoric acid.
  • The collected and derivatized compounds (DNP-hydrazones) were automatically injected into a reversed-phase C18 HPLC column for separation and UV detection at 360 nm.
  • A tandem configuration was employed to increase analysis frequency.

    • Main Results:

    • The automated system successfully sampled and enriched airborne aldehydes and ketones at ng/l concentrations.
    • The method demonstrated effective separation and detection of DNP-hydrazones using HPLC.
    • Optimization of sampling parameters, including reagent concentrations and flow rates, was discussed.

    Conclusions:

    • The developed automated device provides an efficient and effective method for on-line air pollutant sampling and enrichment.
    • This technique facilitates the sensitive determination of aldehydes and ketones in urban air.
    • The automated system offers advantages in terms of speed, convenience, and potentially reduced human error in air quality monitoring.