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Related Experiment Videos

How do stem cells decide what to do?

M A Cross1, C M Heyworth, T M Dexter

  • 1Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.

Ciba Foundation Symposium
|January 1, 1997
PubMed
Summary
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Haemopoietic growth factors support blood cell survival and proliferation. Research suggests lineage commitment involves consolidating pre-existing gene programs rather than external induction in early progenitors.

Area of Science:

  • Hematology
  • Cell Biology
  • Developmental Biology

Background:

  • Mature blood cells are continuously replenished from multipotent stem cells.
  • Haemopoietic growth factors regulate cell survival and proliferation.
  • The role of these factors in lineage choice remains unclear.

Purpose of the Study:

  • To investigate the influence of haemopoietic growth factors on lineage choice in multipotent progenitor cells.
  • To analyze gene expression patterns during lineage commitment.
  • To differentiate the roles of growth factors in early versus lineage-restricted progenitors.

Main Methods:

  • Chromatin analysis of multipotent progenitor cell lines.
  • Analysis of lineage-restricted gene co-expression.

Related Experiment Videos

  • Assessment of growth factor roles in cell survival and lineage commitment in vitro.

    • Main Results:

    • Multipotent progenitor cells maintain genes for alternative fates in an accessible state prior to commitment.
    • Co-expression of lineage-restricted genes suggests commitment consolidates existing programs.
    • Growth factors are necessary for survival but not commitment in multipotential cell lines.
    • Growth factors induce lineage commitment in primary bipotent progenitors.

    Conclusions:

    • Lineage commitment may involve the consolidation of pre-existing, temporally primed gene expression programs.
    • Growth factors play a supportive role in early progenitors but an inductive role in more lineage-restricted cells.