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Related Concept Videos

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Related Experiment Video

Updated: Nov 11, 2025

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
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DNA extraction by zinc

E Kejnovský1, J Kypr

  • 1Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265 Brno, Czech Republic.

Nucleic Acids Research
|May 1, 1997
PubMed
Summary
This summary is machine-generated.

This study presents a rapid DNA extraction method using zinc chloride (ZnCl2) to induce DNA sedimentation. The simple, efficient technique recovers over 90% of DNA in under 30 minutes without costly equipment or hazardous chemicals.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Traditional DNA extraction methods can be time-consuming, expensive, and involve toxic chemicals.
  • There is a need for rapid, simple, and cost-effective DNA isolation techniques for various applications.

Purpose of the Study:

  • To develop and describe a novel, fast, and efficient DNA extraction method.
  • To demonstrate the efficacy of zinc chloride (ZnCl2) induced DNA sedimentation for nucleic acid recovery.

Main Methods:

  • Utilizing millimolar concentrations of zinc chloride (ZnCl2) to induce DNA sedimentation.
  • Employing submillimolar phosphate anion concentrations to enhance zinc-induced DNA precipitation.
  • Assessing DNA recovery rates for both plasmid DNA and short oligonucleotides.

Main Results:

  • The method achieves DNA recovery exceeding 90% within 30 minutes.
  • Effective DNA extraction is demonstrated for concentrations as low as 100 ng/ml for plasmid DNA and 10 microg/ml for oligonucleotides.
  • The procedure requires no expensive facilities or toxic reagents.

Conclusions:

  • This zinc-induced sedimentation method offers a fast, simple, and efficient approach to DNA extraction.
  • The technique is versatile, applicable to various DNA types and concentrations.
  • It presents a cost-effective and safer alternative to conventional DNA isolation protocols.