The Schizosaccharomyces pombe cdc6 gene encodes the catalytic subunit of DNA polymerase delta
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1Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Japan.
Molecular & General Genetics : MGG
|March 18, 1997
Abstract:
The cdc6 mutants of Schizosaccharomyces pombe have been classified as being defective in progression through the G2 phase of the cell cycle. We cloned an S. pombe gene that could complement the temperature-sensitive growth of the cdc6-23 mutant. Unexpectedly, the cloned gene was allelic to pol3, which encodes the catalytic subunit of DNA polymerase delta. Integration mapping confirmed that ccd6 and pol3 are identical. The cdc6-23 mutant carries one amino acid substitution in the conserved N3 region of Pol3.