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Related Experiment Videos

Expansins in deepwater rice internodes

H T Cho1, H Kende

  • 1Michigan State University-Department of Energy Plant Research Laboratory, East Lansing 48824-1312, USA.

Plant Physiology
|April 1, 1997
PubMed
Summary
This summary is machine-generated.

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Deepwater rice cell walls show acid-induced extension, mediated by expansin proteins. Two distinct expansins were identified in rice internodes, contributing to cell wall loosening.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell walls of deepwater rice (Oryza sativa L.) internodes exhibit long-term extension (creep) under tension in acidic conditions.
  • This phenomenon suggests the involvement of cell wall-loosening proteins, specifically expansins.

Purpose of the Study:

  • To investigate the role of expansins in acid-induced cell wall extension in deepwater rice.
  • To identify and characterize the specific expansin proteins present in rice internode cell walls.

Main Methods:

  • Acid-induced cell wall extension assays at varying pH.
  • Boiling experiments to assess heat stability of wall extension.
  • Reconstitution experiments with salt-extracted proteins.
  • Immunoblot analysis using cucumber expansin antibody.

Related Experiment Videos

  • Partial purification of expansins via concanavalin A affinity and SP cation-exchange chromatography.
  • N-terminal amino acid sequencing of purified expansins.

    • Main Results:

    • Cell wall extension demonstrated a pH optimum around 4.0 and was abolished by boiling.
    • Boiled cell walls could be reconstituted with salt-extracted rice or cucumber proteins.
    • Cucumber expansin antibody recognized a 24.5-kD protein band in rice cell walls.
    • Two active expansin fractions (SP20 and SP29) were purified, both containing the 24.5-kD expansin.
    • N-terminal sequencing confirmed the presence of two distinct expansins, Os-EXP1 and Os-EXP2, in rice internodes.

    Conclusions:

    • Two expansins are present in deepwater rice internode cell walls.
    • These expansins likely mediate the observed acid-induced cell wall extension, contributing to plant growth and adaptation.