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T cell signalling through CD73

R Resta1, L F Thompson

  • 1Oklahoma Medical Research Foundation, Immunobiology and Cancer Program, Oklahoma City, USA.

Cellular Signalling
|February 1, 1997
PubMed
Summary
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CD73, an enzyme on T and B cells, helps activate T cells. Its enzymatic activity and anchor are not essential for this activation, but other cell surface molecules are required.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • CD73 (ecto-5'-nucleotidase) is a GPI-anchored enzyme on human lymphocytes.
  • It catalyzes the conversion of nucleoside monophosphates to nucleosides.
  • CD73 plays a role in T cell activation and adenosine generation.

Purpose of the Study:

  • To review the cellular distribution, cDNA sequence, and structure of CD73.
  • To investigate the role of enzymatic activity and GPI anchor in CD73-mediated T cell activation.
  • To discuss signal transmission models for GPI-anchored proteins and CD73's role in adenosine production.

Main Methods:

  • Review of existing literature on CD73.
  • Analysis of a Jurkat T cell line transfected with CD73 cDNA.

Related Experiment Videos

  • Examination of requirements for T cell activation via CD73.

    • Main Results:

    • CD73 functions as a costimulatory molecule for T cell activation.
    • Neither enzymatic activity nor the GPI anchor of CD73 is necessary for in vitro T cell activation.
    • Expression of p56kk, CD45, and the T cell receptor are required for CD73-mediated T cell activation.
    • CD73-generated adenosine has signaling functions in various physiological systems.

    Conclusions:

    • CD73's costimulatory function in T cell activation is independent of its enzymatic activity and GPI anchor.
    • Specific cell surface molecules are crucial for CD73-mediated T cell activation.
    • CD73-derived adenosine is significant for T cell development and other physiological processes.