Yeast colony size reflects YAC copy number
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Updated: Nov 2, 2026
09:04
Yeast Colony Embedding Method
Published on: March 22, 2011
A V Popov1, C Bützler, M Brüggemann
1Department of Development and Genetics, The Babraham Institute, Cambridge CB2 4AT, UK. andrei.popov@bbsrc.ac.uk
Nucleic Acids Research
|May 15, 1997
Abstract:
A novel strategy for separation of co-cloned YACs was developed. For this, yeast cells were grown under non-selective conditions to allow the mitotic loss of multiple YACs. Yeast colonies of different size appear on 'drop-out' selection plates with small clones consistently containing a single-copy YAC. Different auxotrophic marker genes can be used to separate co-cloned YACs or reduce their copy number, which is essential for most YAC-modification procedures.
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